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| Nonino,A.; Nascimento,J.M.; Mascarenhas,C.C.; Mazzeu,J.F.; Pereira,R.W.; Jacomo,R.H.. |
| Myelofibrosis (MF) is characterized by increased circulating hematopoietic progenitor cells (HPCs), abnormal cytokine levels, and the survival advantage of neoplastic progenitors over their normal counterparts, which leads to progressive disappearance of polyclonal hematopoiesis. CD47 is a surface glycoprotein with many functions, such as acting as a phagocytosis inhibitor of the expressing cell, that is increased in normal hematopoietic stem and progenitor cells mobilized into the blood and several human cancer-initiating cells, such as in acute myeloid leukemia. We compared CD47 expression in hematopoietic stem and progenitor cells of patients with MF and controls and found it to be decreased in progenitors of MF. Exposure of control HPCs to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Myeloproliferative disorders; Primary myelofibrosis; Hematopoietic stem cells; Neoplastic stem cells; Antigens; CD47. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100610 |
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| Pranke,P.; Hendrikx,J.; Debnath,G.; Alespeiti,G.; Rubinstein,P.; Nardi,N.; Visser,J.. |
| Identification and enumeration of human hematopoietic stem cells remain problematic, since in vitro and in vivo stem cell assays have different outcomes. We determined if the altered expression of adhesion molecules during stem cell expansion could be a reason for the discrepancy. CD34+CD38- and CD34+CD38+ cells from umbilical cord blood were analyzed before and after culture with thrombopoietin (TPO), FLT-3 ligand (FL) and kit ligand (KL; or stem cell factor) in different combinations: TPO + FL + KL, TPO + FL and TPO, at concentrations of 50 ng/mL each. Cells were immunophenotyped by four-color fluorescence using antibodies against CD11c, CD31, CD49e, CD61, CD62L, CD117, and HLA-DR. Low-density cord blood contained 1.4 ± 0.9% CD34+ cells, 2.6 ± 2.1% of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hematopoietic stem cells; CD34+CD38- cells; Human umbilical cord blood; Ex vivo expansion; Adhesion molecules. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200006 |
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| Canabarro,Raquel; Sporleder,Heloísa; Gomes,Thaís; Zanatta,Geancarlo; Scribel,Letícia; Freitas,Fernando; Neumann,Jorge; Pranke,Patricia. |
| The use of umbilical cord blood stem cells is an efficient alternative for the transplantation of hematopoietic progenitor cells. A number of factors can influence the volume and amount of CD34+ cells, which are considered as immature and capable of proliferation. Quantification of CD34+ cells, evaluation of CD38 and c-kit molecules on these cells, as well as correlations of such factors as maternal age, gestational age, newborn sex and weight, umbilical cord length, placental weight with increased volume and concentration of immature cells, among others, were performed in 70 blood samples from term newborns. The mean volume of umbilical cord blood collected was 53.8±33.6 mL, where 30.96±18.9 CD34+/mL UCB cells were found, of which 16.66±8.32% were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hematopoietic stem cells; Immunophenotyping; Placental/umbilical cord blood. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300006 |
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| Razmkhah,Farnaz; Soleimani,Masoud; Ghasemi,Sorayya; Kafi-abad,Sedigheh Amini. |
| Abstract Microvesicles are able to induce the cell of origin’s phenotype in a target cell. MicroRNA-21, as an oncomir, is up-regulated in almost all cancer types such as leukemia which results in cell proliferation. In this study, we examine the ability of leukemia microvesicles to induce proliferation in hematopoietic stem progenitor cells (HSPCs) via microRNA-21 dysregulation. Herein, leukemia microvesicles were isolated from HL-60 and NB-4 cell lines by ultracentrifugation, and then their protein content was measured. Normal HSPCs were isolated from umbilical cord blood samples by a CD-34 antibody. These cells were treated with 20 and 40 μg/mL leukemia microvesicles for 5 and 10 days, respectively. Cell count, CD-34 analysis, and a microRNA-21 gene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leukemia; Microvesicles; Hematopoietic stem cells; MicroRNA-21. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300465 |
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